Rapid Mass Spectrometric Study of a Supercritical CO2-extract from Woody Liana Schisandra chinensis by HPLC-SPD-ESI-MS/MS

Molecules. 2020 Jun 10;25(11):2689. doi: 10.3390/molecules25112689.


Woody liana Schisandra chinensis contains valuable lignans, which are phenylpropanoids with valuable biological activity. Among green and selective extraction methods, supercritical carbon dioxide (SC-CO2) was shown to be the method of choice for the recovery of these naturally occurring compounds. Carbon dioxide (CO2) was the solvent with the flow rate (10-25 g/min) with 2% ethanol as co-solvent. In this piece of work operative parameters and working conditions were optimized by experimenting with different pressures (200-400 bars) and temperatures (40-60 °C). The extraction time varied from 60 to 120 min. HPLC-SPD-ESI -MS/MS techniques were applied to detect target analytes. Twenty-six different lignans were identified in the S. chinensis SC-CO2 extracts.

Keywords: HPLC-SPD-ESI-MS/MS; Schisandra chinensis; lignans; supercritical fluid extraction.

MeSH terms

  • Carbon Dioxide / analysis*
  • Carbon Dioxide / isolation & purification
  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, Supercritical Fluid / methods*
  • Plant Extracts / chemistry*
  • Schisandra
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Tandem Mass Spectrometry / methods*


  • Plant Extracts
  • Carbon Dioxide