Effect of Angiotensin II on Bone Erosion and Systemic Bone Loss in Mice with Tumor Necrosis Factor-Mediated Arthritis

Int J Mol Sci. 2020 Jun 10;21(11):4145. doi: 10.3390/ijms21114145.


Angiotensin II (Ang II) is the main effector peptide of the renin-angiotensin system (RAS), which regulates the cardiovascular system. The RAS is reportedly also involved in bone metabolism. The upregulation of RAS components has been shown in arthritic synovial tissues, suggesting the potential involvement of Ang II in arthritis. Accordingly, in the present study, we investigated the role of Ang II in bone erosion and systemic bone loss in arthritis. Ang II was infused by osmotic pumps in tumor necrosis factor-transgenic (TNFtg) mice. Ang II infusion did not significantly affect the severity of clinical and histological inflammation, whereas bone erosion in the inflamed joints was significantly augmented. Ang II administration did not affect the bone mass of the tibia or vertebra. To suppress endogenous Ang II, Ang II type 1 receptor (AT1R)-deficient mice were crossed with TNFtg mice. Genetic deletion of AT1R did not significantly affect inflammation, bone erosion, or systemic bone loss. These results suggest that excessive systemic activation of the RAS can be a risk factor for progressive joint destruction. Our findings indicate an important implication for the pathogenesis of inflammatory bone destruction and for the clinical use of RAS inhibitors in patients with rheumatoid arthritis.

Keywords: angiotensin II; angiotensin II type 1 receptor; arthritis; bone erosion; inflammation; renin-angiotensin system; tumor necrosis factor.

MeSH terms

  • Angiotensin II / metabolism*
  • Angiotensin II / pharmacology
  • Animals
  • Arthritis, Experimental / drug therapy
  • Arthritis, Experimental / physiopathology*
  • Bone Resorption / chemically induced
  • Bone Resorption / diagnostic imaging
  • Bone Resorption / etiology*
  • Cancellous Bone / drug effects
  • Cancellous Bone / physiopathology
  • Female
  • Inflammation / drug therapy
  • Inflammation / pathology
  • Joints / metabolism
  • Joints / physiopathology
  • Male
  • Mice, Knockout
  • Mice, Transgenic
  • Receptor, Angiotensin, Type 1 / genetics
  • Receptor, Angiotensin, Type 1 / metabolism
  • Tumor Necrosis Factor-alpha / genetics
  • X-Ray Microtomography


  • Receptor, Angiotensin, Type 1
  • TNF protein, human
  • Tumor Necrosis Factor-alpha
  • Angiotensin II