Glucose Metabolism by Lactobacillus Divergens

J Gen Microbiol. 1988 Aug;134(8):2103-9. doi: 10.1099/00221287-134-8-2103.

Abstract

Earlier studies on the fermentation of D-[1-14C]- and D-[3,4-14C]glucose by Lactobacillus divergens showed that lactate was the major fermentation product and that it was probably produced by glycolysis. It was therefore recommend that L. divergens be reclassified as a homofermentative organism. In the present investigation, products of D-[1-14C]-,D-[2-14C]- and D-[3,4-14C]glucose fermented by L. divergens were isolated, and their specific radioactivities and the distribution patterns of radioactivity in their C-atoms were determined. The positional labelling patterns of the fermentation products, their specific radioactivities and their concentrations confirmed that glucose is degraded via the glycolytic pathway. Some secondary decarboxylation/dissimilation of pyruvate to acetate, formate and CO2 was also observed. These results provide conclusive proof that L. divergens is indeed a homofermentative organism. Results obtained with D-[U-14C]glucose showed that approximately three-quarters of the lactate but less than 10% each of the formate and acetate were produced from glucose. The remainder was presumably derived to a varying degree from endogenous non-glucose sources such as fructose and/or amino acids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates / metabolism
  • Fermentation
  • Glucose / metabolism*
  • Lactates / metabolism
  • Lactic Acid
  • Lactobacillus / metabolism*

Substances

  • Acetates
  • Lactates
  • Lactic Acid
  • Glucose