Laboratory diagnosis of SARS-CoV-2 - A review of current methods

J Infect Public Health. 2020 Jul;13(7):901-905. doi: 10.1016/j.jiph.2020.06.005. Epub 2020 Jun 7.


At present the whole world is facing pandemic of the Coronavirus disease (COVID-19); caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This disease has rapidly spreads across the world from its origin of Wuhan, China and affected millions people worldwide and make them to remain in their homes. The knowledge of available laboratory methods is essential for early and correct diagnosis of COVID-19 to identify new cases as well as monitoring treatment of confirmed cases. In this review we aim to provide the updated information about selection of specimens and availability of various diagnostic methods and their utility with current findings for the laboratory diagnosis of SARS-CoV-2 infection. This will guide the healthcare professionals and government organizations to make strategy for establishing diagnostic facilities for SARS-CoV-2 infections.

Keywords: COVID-19; Corona virus; Immunological assay; Lab diagnosis; RT-PCR.

Publication types

  • Review

MeSH terms

  • Betacoronavirus / isolation & purification*
  • COVID-19
  • Coronavirus Infections / diagnosis*
  • Coronavirus Infections / prevention & control
  • Coronavirus Infections / virology
  • Humans
  • Immunoassay / methods*
  • Nasopharynx / virology
  • Pandemics / prevention & control
  • Pneumonia, Viral / diagnosis*
  • Pneumonia, Viral / prevention & control
  • Pneumonia, Viral / virology
  • Real-Time Polymerase Chain Reaction / methods*
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • SARS-CoV-2
  • Specimen Handling
  • Virus Cultivation / methods*