Applications of small molecules in modulating productivity and product quality of recombinant proteins produced using cell cultures

Abstract

Mammalian cell cultures have been used extensively for production of recombinant protein therapeutics such as monoclonal antibodies, fusion proteins and enzymes for decades. Small molecules have been investigated as media supplements to improve process productivity and reduce cost of goods. Those chemicals can lead to significant yield improvement through different mechanisms such as cell cycle modulation, cellular redox regulation, etc. In addition to productivity, small molecules have also been routinely used to regulate post-translational modifications of recombinant proteins. This review summarizes key applications of small molecules in protein productivity improvement and product quality control.

Keywords: Biologics production; Cell culture media supplements; Cell cycle; Cell growth; Charge heterogeneity; Chemicals; Chinese hamster ovary; Glycosylation; Protein quality; Small molecule.