A Guide to the Development of Human CorneaOrganoids from Induced Pluripotent Stem Cells in Culture

Methods Mol Biol. 2020:2145:51-58. doi: 10.1007/978-1-0716-0599-8_5.


The cornea is the outermost transparent and refractive barrier surface of the eye necessary for vision. Development of the cornea involves the coordinated production of extracellular matrix, epithelial differentiation, and endothelial cell expansion to produce a highly transparent tissue. Here we describe the production of multilayered three-dimensional organoids from human-induced pluripotent stem cells. These organoids have the potential for multiple downstream applications which are currently unattainable using traditional in vitro techniques.

Keywords: Cornea; Corneal epithelium; Corneal stroma; Organoids; iPSC.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Culture Techniques / methods*
  • Cell Differentiation / genetics
  • Cell Proliferation / genetics
  • Cornea / cytology
  • Cornea / growth & development
  • Epithelium, Corneal / cytology*
  • Epithelium, Corneal / growth & development
  • Extracellular Matrix / genetics
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Organoids / cytology*
  • Organoids / growth & development