Genome-wide sequence identification and expression analysis of ARF family in sugar beet (Beta vulgaris L.) under salinity stresses

Jie Cui; Xinyan Li; Junliang Li; Congyu Wang; Dayou Cheng; Cuihong Dai

doi:10.7717/peerj.9131

Genome-wide sequence identification and expression analysis of ARF family in sugar beet (Beta vulgaris L.) under salinity stresses

PeerJ. 2020 Jun 4:8:e9131. doi: 10.7717/peerj.9131. eCollection 2020.

Authors

Jie Cui¹, Xinyan Li¹, Junliang Li¹, Congyu Wang¹, Dayou Cheng¹, Cuihong Dai¹

Affiliation

¹ Harbin Institute of Technology, Harbin, China.

Abstract

Auxin response factor (ARF) proteins respond to biological and abiotic stresses and play important roles in regulating plant growth and development. In this study, based on the genome-wide database of sugar beet, 16 BvARF proteins were identified. A detailed investigation into the BvARF family is performed, including analysis of the conserved domains, chromosomal locations, phylogeny, exon-intron structure, conserved motifs, subcellular localization, gene ontology (GO) annotations and expression profiles of BvARF under salt-tolerant condition. The majority of BvARF proteins contain B3 domain, AUX_RESP domain and AUX/IAA domain and a few lacked of AUX/IAA domain. Phylogenetic analysis suggests that the 16 BvARF proteins are clustered into six groups. Expression profile analysis shows that most of these BvARF genes in sugar beet under salinity stress were up-regulated or down-regulated to varying degrees and nine of the BvARF genes changed significantly. They were thought to have a significant response to salinity stress. The current study provides basic information for the BvARF genes and will pave the way for further studies on the roles of BvARF genes in regulating sugar beet's growth, development and responses to salinity stress.

Keywords: ARF; Bioinformation; Salinity stress; Sugar beet.

Grants and funding

This work was supported by the National Natural Science Foundation of China (No. 31571731), and the Sugar Industry Modern Industrial Technology System Construction Project of Ministry of Agriculture (NO. CARS-170111-06 and NO. CARS-170104-01). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.