Endothelial nitric oxide synthase gene polymorphisms and erectile dysfunction in chronic pain

Ana Segura; Pura Ballester; Raquel Ajo; María-Del-Mar Inda; Antonio Urbano; Javier Muriel; Isabel Ochando; César Margarit; Emi Martinez; Ana M Peiró

doi:10.1016/j.gene.2019.100005

Endothelial nitric oxide synthase gene polymorphisms and erectile dysfunction in chronic pain

Gene X. 2019 Feb 2:1:100005. doi: 10.1016/j.gene.2019.100005. eCollection 2019 Feb.

Authors

Ana Segura^{1

2}, Pura Ballester², Raquel Ajo², María-Del-Mar Inda², Antonio Urbano^{3

4}, Javier Muriel^{2

5}, Isabel Ochando^{3

4}, César Margarit^{2

6}, Emi Martinez⁶, Ana M Peiró^{2

7}

Affiliations

¹ Andrology Unit, University General Hospital of Alicante (HGUA), Alicante, Spain.
² Neuropharmacology on Pain Research Unit, Institute of Health and Biomedical Research of Alicante (ISABIAL-FISABIO), Alicante, Spain.
³ Genetics Unit, Clínica Vistahermosa HLA-Hospital, Alicante, Spain.
⁴ Histology and Anatomy Department, Miguel Hernández University (UMH), Alicante, Spain.
⁵ Occupational Observatory, University Miguel Hernández of Elche (UMH), Alicante, Spain.
⁶ Pain Unit, HGUA, Alicante, Spain.
⁷ Clinical Pharmacology, HGUA, Alicante, Spain.

Abstract

Objectives: To investigate whether endothelial nitric oxide synthase (eNOS) T786C, 4VNTR and G894 T gene polymorphisms could mediate in andrological treatment response in Spaniards.

Subject patients/methods: The study participants were Spaniard males with erectile dysfunction (ED) and chronic pain (n = 105) recruited at the Pain Unit. eNOS polymorphisms were genotyped by quantitative polymerase chain reaction using Taqman specific probes. Statistical analyses were carried out using R-3.2.4 software.

Results: A total of 69 patients required andrological treatment and 76% of them improved ED upon iPED5 (20%), testosterone (35%) or iPDE5/testosterone treatment (45%); being significantly better in T786C-CC patients. Multivariate regression analysis indicated that age, opioid daily dose and carriage of T786C-C allele influenced the risk and ED severity in Spaniard chronic pain patients.

Conclusion: T786C polymorphism at eNOS locus appeared to be a major contributor in the variable erectile function iPDE5/testosterone response in Spaniards.

Keywords: BMI, body mass index; CNP, chronic non-cancer pain; Chronic pain; ED, erectile dysfunction; EF, Erectile function; Erectile dysfunction; IIEF, International Index of Erectile Function; NO, nitric oxide; Pharmacogenetics; T786C; VAS, Visual analogue scale; cGMP, 3′,5′-cyclic guanosine monophosphate; eNOS gene; eNOS, endothelial nitric oxide synthase; iPDE5; iPDE5, phosphodiesterase type 5 inhibitors; mSLQQ-QOL, modified Sexual Life Quality Questionnaire.