Acetyl-α-boswellic acid and Acetyl-β-boswellic acid protects against caerulein-induced pancreatitis via down-regulating MAPKs in mice

Int Immunopharmacol. 2020 Sep;86:106682. doi: 10.1016/j.intimp.2020.106682. Epub 2020 Jun 18.

Abstract

This study is to investigate the protective effect of Acetyl-α-boswellic acid and Acetyl-β-boswellic mixture(α/β-ABA), which is the active ingredients isolated from Frankincense, on actue pancreatitis and its mechanism. Our experimental results showed that 2 μM α/β-ABA reduced production of NO, TNF-α, IL-6, IL-10 and IL-1β in RAW264.7 cells that were stimulated with lipopolysaccharide (LPS) which indicates its anti-inflammatory role. In pancreatitis model induced by caerulein, intra-gastrical administration of 100 mg/kg α/β-ABA relieved inflammatory cells infiltration significantly and attenuated the serum elevation of amylase TNF-α and IL-6 remarkably in mice. Furthermore, α/β-ABA down-regulated mitogen-activated protein kinase (MAPK) family phosphorylated proteins in pancreas, including phosphorylated p38, ERK1/2 and JNK, to reduce the serum inflammatory factors. Finally, α/β-ABA alleviated the pancreatic edema and inflammatory cell infiltration in pancreatitis mice model. This study suggests that α/β-ABA may be targeted for drug development against pancreatitis via modulating MAPKs pathway.

Keywords: Acetyl-α-boswellic acid; Acetyl-β-boswellic acid; ERK; JNK; Pancreatitis; p38.

MeSH terms

  • Animals
  • Cell Survival / drug effects
  • Ceruletide / toxicity
  • Cytokines / drug effects
  • Cytokines / metabolism
  • Disease Models, Animal
  • Down-Regulation / drug effects
  • Edema / drug therapy
  • Inflammation / drug therapy
  • Lipopolysaccharides / toxicity
  • Mice
  • Mice, Inbred C57BL
  • Mitogen-Activated Protein Kinases / genetics*
  • Pancreatitis / chemically induced
  • Pancreatitis / pathology
  • Pancreatitis / prevention & control*
  • RAW 264.7 Cells
  • Triterpenes / pharmacology*
  • Triterpenes / therapeutic use

Substances

  • 3-O-acetyl-beta-boswellic acid
  • Cytokines
  • Lipopolysaccharides
  • Triterpenes
  • Ceruletide
  • Mitogen-Activated Protein Kinases