A novel method for nucleic acid sequence determination

J Theor Biol. 1988 Dec 7;135(3):303-7. doi: 10.1016/s0022-5193(88)80246-7.


We describe a novel sequencing methodology which should be readily and completely automated. The method relies on fragmentation of a nucleotide or deoxynucleotide sequence into short fragments, and subsequent quantitation of the fragments by hybridization to oligo-deoxynucleotides on a solid support. The original sequence may be reconstructed from the resulting table of fragment frequencies. We present a specific protocol which would allow practical implementation of this approach.

MeSH terms

  • Base Sequence*
  • Methods
  • Nucleic Acid Hybridization*
  • Repetitive Sequences, Nucleic Acid