Bacteriophage-Antibiotic Combinations for Enterococcus faecium With Varying Bacteriophage and Daptomycin Susceptibilities

Antimicrob Agents Chemother. 2020 Jun 22;AAC.00993-20. doi: 10.1128/AAC.00993-20. Online ahead of print.

Abstract

Concerns regarding increased prevalence of daptomycin (DAP)-resistant strains necessitate novel therapies for Enterococcus faecium infections. Obligately lytic bacteriophages are viruses that target, infect, and kill bacterial cells. Limited studies have evaluated phage-antibiotic combinations against E. faecium Following an initial screen of eight E. faecium strains, three strains with varying DAP/phage susceptibility were selected for further experiments. Phage-to-strain specificity contributed to synergy with antibiotics by time-kill analyses and was associated with lower development of phage resistance.