Early appearance of activated CD4+ T lymphocytes and class II antigen-expressing cells in joints of DBA/1 mice immunized with type II collagen

Lab Invest. 1988 Jan;58(1):53-60.


Arthritis induced with type II collagen in DBA/1 mice, was analyzed by immunohistochemical techniques. In the earliest detectable pathologic changes, before any macroscopic signs, an accumulation of Mac1+, macrophage-like cells, and an increased expression of major histocompatibility class II antigens were observed focally in the synovial lining layer. In these foci, CD4+ and interleukin 2 receptor expressing T lymphocytes were regularly detected, but not usually other sets of lymphocytes such as B lymphocytes and CD8+ lymphocytes. In clinically detectable arthritis, there was a prominent infiltration of Mac1+ cells, both polymorphonuclear-like and macrophage-like cells. T cells were relatively few, suggesting that they do not play a primary effector role, but rather that they may regulate or permit the self-perpetuative inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Differentiation, T-Lymphocyte / analysis*
  • Arthritis / pathology*
  • Arthritis, Experimental / etiology
  • Arthritis, Experimental / metabolism
  • Arthritis, Experimental / pathology*
  • Collagen / administration & dosage*
  • Collagen / immunology
  • Histocompatibility Antigens Class II / analysis*
  • Immunohistochemistry
  • Inflammation / etiology
  • Inflammation / metabolism
  • Inflammation / pathology
  • Joints / analysis
  • Joints / pathology
  • Lymphocyte Activation*
  • Male
  • Mice
  • Mice, Inbred DBA
  • T-Lymphocytes / immunology
  • T-Lymphocytes / pathology*
  • Time Factors


  • Antigens, Differentiation, T-Lymphocyte
  • Histocompatibility Antigens Class II
  • Collagen