T lymphocytes compartmentalized on the epithelial surface of the lower respiratory tract express the very late activation antigen complex VLA-1

Clin Immunol Immunopathol. 1988 Feb;46(2):221-33. doi: 10.1016/0090-1229(88)90185-7.


T lymphocytes on the epithelial surface of the lower respiratory tract are thought to represent a relatively compartmentalized population of T cells that exchanges slowly with the blood. Since the lung is chronically burdened with antigens, "resident" T cells likely have a history of past activation. To evaluate this concept, we analyzed resident lung T cells for VLA-1 expression, which is indicative of a history of past stimulation. Lung lavage and blood T cells were evaluated in 13 normal nonsmokers using the monoclonal antibodies Leu4 (pan T cells), Leu3 (helper/inducer T cells), Leu2 (suppressor/cytotoxic T cells), TS2/7 (alpha 1 subunit of VLA-1), and A-1A5 (beta subunit of VLA-1) using immunofluorescence and immunoprecipitation. In contrast to normal blood T cells which did not express VLA-1, lung T cells expressed the 210-kDa alpha 1 and 130-kDa beta subunits of the VLA-1 complex, the same as blood T cells activated in culture for 3 weeks. Two-color immunofluorescence with Leu4 and TS2/7 showed that 19 +/- 6% of the lung T cells were VLA-1+, suggesting that a significant proportion of T lymphocytes on the alveolar epithelial surface are in a separate compartment from the VLA-1 blood cells. In sarcoidosis, a disease characterized by exaggerated numbers of active Leu3+ T cells in the lower respiratory tract, increased numbers of lung Leu3+ T cells expressing VLA-1 were present on the epithelial surface of the lung (P less than 0.05 compared to normals). These observations are consistent with compartmentalized, chronically stimulated T lymphocytes on the alveolar epithelial surface that exchange with the systemic immune system very slowly.

MeSH terms

  • Antibodies, Monoclonal
  • Antigens, Differentiation / immunology*
  • Epithelium / immunology
  • Female
  • Lung / immunology*
  • Lymphocyte Activation*
  • Male
  • Pulmonary Alveoli / immunology
  • Receptors, Very Late Antigen
  • Sarcoidosis / immunology*
  • Smoking / immunology
  • T-Lymphocytes / immunology*


  • Antibodies, Monoclonal
  • Antigens, Differentiation
  • Receptors, Very Late Antigen