In transgenic mice the introduced functional T cell receptor beta gene prevents expression of endogenous beta genes

Cell. 1988 Mar 25;52(6):831-41. doi: 10.1016/0092-8674(88)90425-4.


Transgenic mice were constructed with a functional T cell receptor beta gene. Transcription of the introduced gene is largely confined to T cells, but low levels of transcripts are also seen in B cells and in other tissues. Serological analyses show that most, if not all, of the T lymphocytes express the transgenic beta chain on the cell surface and lack beta chains encoded by endogenous beta genes. Molecular genetic analyses of uncloned and cloned T lymphocytes demonstrate that rearrangement of endogenous beta genes is incomplete. Partial D beta 1-J beta 1 rearrangements are found preferentially, while complete VDJ rearrangements are not seen. These findings show that expression of the transgene regulates the rearrangement of endogenous beta genes. Although the alpha beta T cell receptors of the transgenic mice are homogeneous with respect to the beta chain, they are fully functional, at least in a variety of allogeneic responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Membrane / immunology
  • Cells, Cultured
  • Clone Cells
  • Cytotoxicity, Immunologic
  • Female
  • Gene Expression Regulation*
  • Genes*
  • Male
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Receptors, Antigen, T-Cell / genetics*
  • T-Lymphocytes / immunology
  • T-Lymphocytes, Cytotoxic / immunology
  • Transcription, Genetic*


  • Receptors, Antigen, T-Cell

Associated data

  • GENBANK/M19404