Oligonucleotide probes (TEM-1, OXA-1) versus isoelectric focusing in beta-lactamase characterization of 114 resistant strains

M Ouellette; G C Paul; A M Philippon; P H Roy

doi:10.1128/AAC.32.3.397

Oligonucleotide probes (TEM-1, OXA-1) versus isoelectric focusing in beta-lactamase characterization of 114 resistant strains

Antimicrob Agents Chemother. 1988 Mar;32(3):397-9. doi: 10.1128/AAC.32.3.397.

Authors

M Ouellette¹, G C Paul, A M Philippon, P H Roy

Affiliation

¹ Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Ste-Foy, Quebec, Canada.

Abstract

Oligonucleotide probes specific for detection of the TEM-1 and OXA-1 beta-lactamase genes were compared with isoelectric focusing in 114 gram-negative beta-lactamase-producing strains representing at least 16 species. Correlations of 96 and 100% with isoelectric points were found for the TEM-1 and OXA-1 probes, respectively.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / enzymology*
Drug Resistance, Microbial
Isoelectric Focusing
Oligonucleotides*
beta-Lactamases / analysis*

Substances

Oligonucleotides
beta-Lactamases