Characterization and Functional Properties of a Novel Monoclonal Antibody Which Identifies a T Cell Receptor in Chickens

Eur J Immunol. 1988 Apr;18(4):533-7. doi: 10.1002/eji.1830180407.

Abstract

A monoclonal antibody, mAb6, was produced that specifically bound to chicken T lymphocytes. Immunofluorescence analysis using a fluorescence-activated cell sorter revealed that the antibody reacted with approximately 50% of blood lymphocytes and with approximately 40% of splenocytes and thymocytes. It did not react with bursal cells and erythrocytes. Among different types of hemopoietic cell lines, only a T cell line was reactive with mAb6. When coupled to Sepharose 4B beads, mAb6 was found to be highly mitogenic for chicken T cells. In soluble form, mAb6 inhibited concanavalin A-induced T cell proliferation and the cytotoxic activity of allosensitized T cells, the inhibition occurring in a dose-dependent manner for both assays. Thus, the tissue distribution and the effects of this antibody on T cell responses suggest that mAb6 recognizes a T cell receptor present on a large proportion of chicken T cells.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antigen-Antibody Reactions
  • Cell Separation
  • Chickens / immunology*
  • Concanavalin A / pharmacology
  • Flow Cytometry
  • Hematopoietic Stem Cells / immunology
  • Lymphocyte Activation
  • Receptors, Antigen, T-Cell / immunology*
  • T-Lymphocytes / classification
  • T-Lymphocytes / immunology*
  • T-Lymphocytes, Cytotoxic / immunology
  • Tissue Distribution

Substances

  • Antibodies, Monoclonal
  • Receptors, Antigen, T-Cell
  • Concanavalin A