Development of optimal steam explosion pretreatment and highly effective cell factory for bioconversion of grain vinegar residue to butanol

Menglei Xia; Mingmeng Peng; Danni Xue; Yang Cheng; Caixia Li; Di Wang; Kai Lu; Yu Zheng; Ting Xia; Jia Song; Min Wang

doi:10.1186/s13068-020-01751-7

Development of optimal steam explosion pretreatment and highly effective cell factory for bioconversion of grain vinegar residue to butanol

Biotechnol Biofuels. 2020 Jun 24:13:111. doi: 10.1186/s13068-020-01751-7. eCollection 2020.

Authors

Menglei Xia^{1

2}, Mingmeng Peng^{1

2}, Danni Xue^{1

2}, Yang Cheng^{1

2}, Caixia Li^{1

2}, Di Wang^{1

2}, Kai Lu^{1

2}, Yu Zheng^{1

2}, Ting Xia^{1

2}, Jia Song^{1

2}, Min Wang^{1

2}

Affiliations

¹ State Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457 China.
² Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457 China.

Abstract

Background: The industrial vinegar residue (VR) from solid-state fermentation, mainly cereals and their bran, will be a potential feedstock for future biofuels because of their low cost and easy availability. However, utilization of VR for butanol production has not been as much optimized as other sources of lignocellulose, which mainly stem from two key elements: (i) high biomass recalcitrance to enzymatic sugar release; (ii) lacking of suitable industrial biobutanol production strain. Though steam explosion has been proved effective for bio-refinery, few studies report SE for VR pretreatment. Much of the relevant knowledge remains unknown. Meanwhile, recent efforts on rational metabolic engineering approaches to increase butanol production in Clostridium strain are quite limited. In this study, we assessed the impact of SE pretreatment, enzymatic hydrolysis kinetics, overall sugar recovery and applied atmospheric and room temperature plasma (ARTP) mutant method for the Clostridium strain development to solve the long-standing problem.

Results: SE pretreatment was first performed. At the optimal condition, 29.47% of glucan, 71.62% of xylan and 22.21% of arabinan were depolymerized and obtained in the water extraction. In the sequential enzymatic hydrolysis process, enzymatic hydrolysis rate was increased by 13-fold compared to the VR without pretreatment and 19.60 g glucose, 15.21 g xylose and 5.63 g arabinose can be obtained after the two-step treatment from 100 g VR. Porous properties analysis indicated that steam explosion can effectively generate holes with diameter within 10-20 nm. Statistical analysis proved that enzymatic hydrolysis rate of VR followed the Pseudop-second-order kinetics equation and the relationship between SE severity and enzymatic hydrolysis rate can be well revealed by Boltzmann model. Finally, a superior inhibitor-tolerant strain, Clostridium acetobutylicum Tust-001, was generated with ARTP treatment. The water extraction and enzymolysis liquid gathered were successfully fermented, resulting in butanol titer of 7.98 g/L and 12.59 g/L of ABE.

Conclusions: SE proved to be quite effective for VR due to high fermentable sugar recovery and enzymatic hydrolysate fermentability. Inverse strategy employing ARTP and repetitive domestication for strain breeding is quite feasible, providing us with a new tool for solving the problem in the biofuel fields.

Keywords: Bioconversion; Industrial vinegar residue; Integration of ARTP and repetitive domestication; Kinetic model; Steam explosion; Working mechanism.