Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0

Synth Syst Biotechnol. 2020 Jun 13;5(2):99-102. doi: 10.1016/j.synbio.2020.05.005. eCollection 2020 Jun.

Abstract

CRISPR/Cas9 systems are an established tool in genome engineering. As double strand breaks caused by the standard Cas9-based knock-out techniques can be problematic in some organisms, new systems were developed that can efficiently create knock-outs without causing double strand breaks to elegantly sidestep these issues. The recently published CRISPR-BEST base editor system for actinobacteria is built around a C to T or A to G base exchange. These base editing systems however require additional constraints to be considered for designing the sgRNAs. Here, we present an updated version of the interactive CRISPy-web single guide RNA design tool https://crispy.secondarymetabolites.org/that was built to support "classical" CRISPR and now also CRISPR-BEST workflows.

Keywords: Base editor; CRISPR; CRISPR-BEST; Genome editing; Webserver; sgRNA.