Protein adsorption and in vitro behavior of additively manufactured 3D-silicon nitride scaffolds intended for bone tissue engineering

Abstract

Highly porous scaffolds of Si₃N₄ are fabricated by direct ink writing method (Robocasting) with a pattern of macroporous cavities of 650-700μm. Two different Si₃N₄ ink compositions regarding the oxide sintering aids (namely, Y₂O₃, Al₂O₃, and SiO₂) are tried. Both inks reach solid volume fractions of ~0.40 with about 10-12wt% of polymeric additive content that imparts the necessary pseudoplastic characteristics. The printed structures are sintered under controlled N₂ atmosphere either in a conventional graphite furnace or by the spark plasma sintering technique. Skeleton of the scaffolds reaches densities above 95% of the theoretical value with ≈18-24% of linear shrinkage. Analysis of the crystalline phases, microstructure and mechanical properties are comparatively done for both compositions. The bioactivity of these structures is addressed by evaluating the ion release rate in simulated body fluid. In parallel, atomic force microscopy is used to determine the effect of the filaments surface roughness on protein adsorption (Bovine Serum Albumin) for assessing the potential application of 3D-Si₃N₄ scaffolds in bone regeneration.

Keywords: 3D printing; Bioactivity; Bone scaffolds; Protein adsorption; Silicon nitride.