Complete Amino Acid Sequence of p453-plasmid-mediated PIT-2 Beta-Lactamase (SHV-1)

Biochem J. 1988 Apr 1;251(1):73-9. doi: 10.1042/bj2510073.

Abstract

The complete amino acid sequence of the p453-plasmid-mediated PIT-2 beta-lactamase (SHV-1) was determined. The protein contains 265 residues. Peptides resulting from digestions with trypsin, Staphylococcus aureus V8 proteinase, chymotrypsin and Lys-C proteinase and cleavage with CNBr were separated and purified by using reverse-phase h.p.l.c. The amino acid sequence of each peptide was manually determined with the dimethylaminoazobenzene isothiocyanate/phenyl isothiocyanate double-coupling method. The primary structure of PIT-2 beta-lactamase was compared with those of two closely related enzymes, namely TEM-1 beta-lactamase and the beta-lactamase of Klebsiella pneumoniae strain LEN-1. The PIT-2 beta-lactamase amino acid sequence was strongly retained, with respectively 68% and 88% homology. Thus PIT-2 enzyme could represent an evolutionary step between a chromosomally encoded beta-lactamase and the plasmid-mediated TEM beta-lactamases.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Chromatography, High Pressure Liquid
  • Molecular Sequence Data
  • Peptide Fragments / isolation & purification
  • Plasmids*
  • beta-Lactamases*

Substances

  • Peptide Fragments
  • beta-lactamase PIT-2
  • beta-Lactamases