Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model

Qiushi Tang; Gwladys Gernoux; Yangfun Cheng; Terence Flotte; Christian Mueller

doi:10.1007/978-1-0716-0704-6_9

Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model

Methods Mol Biol. 2020:2164:75-85. doi: 10.1007/978-1-0716-0704-6_9.

Authors

Qiushi Tang¹, Gwladys Gernoux², Yangfun Cheng^{1

3}, Terence Flotte⁴, Christian Mueller⁵

Affiliations

¹ Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA.
² Department of Pediatrics, Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA.
³ West China School of Medicine, Sichuan University, Chengdu, China.
⁴ Microbiology and Physiologic Systems, Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA.
⁵ Department of Pediatrics, Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA. chris.mueller@umassmed.edu.

PMID: 32607885
DOI: 10.1007/978-1-0716-0704-6_9

Abstract

We recently described the generation of a novel mouse strain that efficiently and readily engrafts human primary hepatocytes to create liver xenografts (Borel et al., Mol Ther, 25: 2477-89, 2017). A transgenic mouse strain expressing a human PiZ allele for the SerpinA1 gene was crossed with the NOD-SCID-gamma chain knockout (NSG) strain to create a recipient strain (PiZ-NSG) for human hepatocyte xenotransplantation. In this chapter we provide a description of the methods to achieve these liver xenografts in the PiZ-NSG mouse.

Keywords: Engraftment; Hepatectomy; Hepatocyte isolation; Human hepatocyte; Humanized mouse model; Intrasplenic injection; Liver regeneration; Liver xenograft.

MeSH terms

Alleles
Animals
Disease Models, Animal
Hepatocytes / transplantation*
Humans
Mice
Mice, Inbred NOD
Mice, Knockout
Mice, SCID
Mice, Transgenic
Transplantation, Heterologous / methods
alpha 1-Antitrypsin / genetics

Substances

alpha 1-Antitrypsin