Accessory cells, dendritic cells, or monocytes, are required for the lymphokine-activated killer cell induction from resting T cell but not from natural killer cell precursors

J Immunol. 1988 Aug 15;141(4):1404-9.

Abstract

In this study we have investigated the role of accessory cells in the development of lymphokine-activated killer cells (LAK) from highly purified human NK and small resting T cell progenitors. As accessory cells we used autologous, as well as allogeneic, monocytes, and dendritic cell enriched cells. Both NK and T cells were able to generate LAK activity, but their activation requirements were different. NK cells were activated merely by IL-2, and accessory cells did not enhance their lytic activity in the presence or absence of IL-2. Conversely, T cells were practically unresponsive to even high concentrations of IL-2 having a strict requirement for accessory cells for the development of lytic activity and proliferation. Accessory cells differed in their ability to activate T cells presumably depending on their ability to induce IL-2 synthesis, allogeneic dendritic cells being the most effective accessory cells and IL-2 synthesis stimulators. Allogeneic accessory cells could induce lytic activity in T cells even in the absence of exogenous IL-2. Thus, accessory cells play a central role in expanding the LAK effector cell population.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigen-Presenting Cells / immunology*
  • Antigen-Presenting Cells / physiology
  • Dendritic Cells / immunology*
  • Dendritic Cells / physiology
  • Humans
  • Interphase
  • Killer Cells, Natural / immunology*
  • Killer Cells, Natural / physiology
  • Leukocyte Count
  • Lymphocyte Activation*
  • Lymphokines
  • Monocytes / immunology*
  • Monocytes / physiology
  • Stem Cells / immunology
  • Stem Cells / physiology
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / physiology

Substances

  • Lymphokines