Fluctuating expression of miR-584 in primary and high-grade gastric cancer

BMC Cancer. 2020 Jul 2;20(1):621. doi: 10.1186/s12885-020-07116-5.


Background: Gastric cancer is the fifth most common cancer worldwide. Along with environmental factors, such as Helicobacter pylori (H. pylori) infection, genetic changes play important roles in gastric tumor formations. miR-584 is a less well-characterized microRNA (miRNA), with apparent activity in human cancers. However, miR-584 expression pattern in gastric cancer development has remained unclear. This study aims to analyze the expression of miR-584 in gastric cancer samples and investigates the associations between this miRNA and H. pylori infection and clinical characteristics.

Methods: The expression level of miR-584 was studied in primary gastric cancers versus healthy control gastric mucosa samples using the RT-qPCR method. The clinical data were analyzed statistically in terms of miR-584 expression. In silico studies were employed to study miR-584 more broadly in order to assess its expression and find new potential target genes.

Results: Both experimental and in silico studies showed up-regulation of miR-584 in patients with gastric cancer. This up-regulation seems to be induced by H. pylori infection since the infected samples showed increased levels of miR-584 expression. Deeper analyses revealed that miR-584 undergoes a dramatic down-regulation in late stages, invasive and lymph node-metastatic gastric tumors. Bioinformatics studies demonstrated that miR-584 has a substantial role in cancer pathways and has the potential to target STAT1 transcripts. Consistent with the inverse correlation between TCGA RNA-seq data of miR-584 and STAT1 transcripts, the qPCR analysis showed a significant negative correlation between these two RNAs in a set of clinical samples.

Conclusion: miR-584 undergoes up-regulation in the stage of primary tumor formation; however, becomes down-regulated upon the progression of gastric cancer. These findings suggest the potential of miR-584 as a diagnostic or prognostic biomarker in gastric cancer.

Keywords: Computational analysis; Gastric cancer; Helicobacter pylori; miR-584.

MeSH terms

  • Adult
  • Aged
  • Biomarkers, Tumor / metabolism*
  • Case-Control Studies
  • Computational Biology
  • Down-Regulation
  • Female
  • Gastric Mucosa / microbiology
  • Gastric Mucosa / pathology
  • Gene Expression Regulation, Neoplastic*
  • Helicobacter Infections / diagnosis
  • Helicobacter Infections / genetics*
  • Helicobacter Infections / microbiology
  • Helicobacter Infections / pathology
  • Helicobacter pylori / isolation & purification
  • Host-Pathogen Interactions / genetics
  • Humans
  • Kaplan-Meier Estimate
  • Male
  • MicroRNAs / metabolism*
  • Middle Aged
  • Neoplasm Grading
  • Prognosis
  • RNA-Seq
  • STAT1 Transcription Factor / genetics
  • Stomach Neoplasms / diagnosis
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / microbiology
  • Stomach Neoplasms / mortality
  • Survival Rate
  • Up-Regulation


  • Biomarkers, Tumor
  • MIRN584 microRNA, human
  • MicroRNAs
  • STAT1 Transcription Factor
  • STAT1 protein, human