Generation of urine-derived iPS cell line via a non-integrative method from a Barth syndrome patient with TAZ gene mutation

Xiaoling Guo; Long Wang; Kaixin Chen; Shiyang Song; Xingang Wang; Xiaohong Gu; Chao Niu; Maoping Chu

doi:10.1016/j.scr.2020.101886

Generation of urine-derived iPS cell line via a non-integrative method from a Barth syndrome patient with TAZ gene mutation

Stem Cell Res. 2020 Jun 24:47:101886. doi: 10.1016/j.scr.2020.101886. Online ahead of print.

Authors

Xiaoling Guo¹, Long Wang², Kaixin Chen³, Shiyang Song⁴, Xingang Wang³, Xiaohong Gu³, Chao Niu⁴, Maoping Chu⁵

Affiliations

¹ Center of Scientific Research, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China; Institute of Cardiovascular Development and Translational Medicine, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China. Electronic address: guoxling@hotmail.com.
² Department of Cardiology, The Affiliated Hangzhou First People's Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
³ Center of Scientific Research, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China; Institute of Cardiovascular Development and Translational Medicine, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
⁴ Institute of Cardiovascular Development and Translational Medicine, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
⁵ Center of Scientific Research, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China; Institute of Cardiovascular Development and Translational Medicine, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China. Electronic address: chmping@hotmail.com.

PMID: 32619718
DOI: 10.1016/j.scr.2020.101886

Abstract

Human urine cells from a 6-year-old male X-linked Barth syndrome patient harboring a TAZ frameshift (c.517delG, Xq28) were reprogrammed into the induced pluripotent stem cell (iPSC) line WMUi002-A using non-integration CytoTune®-iPS 2.0 Sendai Virus Reprogramming kit, including four well-known Yamanaka factors SOX2, OCT4, KLF4, and c-MYC. The established patient-derived iPSC expressed endogenous pluripotent markers, had the potential to differentiate into all of the three germ layers, and displayed a normal karyotype.