TH17/Treg imbalance and IL-17A increase after severe aneurysmal subarachnoid hemorrhage

Leandro Moraes; Natalia Trias; Andreina Brugnini; Pedro Grille; Daniela Lens; Alberto Biestro; Sofia Grille

doi:10.1016/j.jneuroim.2020.577310

TH17/Treg imbalance and IL-17A increase after severe aneurysmal subarachnoid hemorrhage

J Neuroimmunol. 2020 Jun 27:346:577310. doi: 10.1016/j.jneuroim.2020.577310. Online ahead of print.

Authors

Leandro Moraes¹, Natalia Trias², Andreina Brugnini², Pedro Grille³, Daniela Lens², Alberto Biestro⁴, Sofia Grille⁵

Affiliations

¹ Cátedra de Medicina Intensiva, Hospital de Clínicas, Facultad de Medicina, Universidad de la República, Av Italia s/n, 11300 Montevideo, Uruguay; Laboratorio de Citometria y Biologia Molecular, Departamento Básico de Medicina, Hospital de Clínicas, Facultad de Medicina, Universidad de la República, Av Italia s/n, 11300 Montevideo, Uruguay.
² Laboratorio de Citometria y Biologia Molecular, Departamento Básico de Medicina, Hospital de Clínicas, Facultad de Medicina, Universidad de la República, Av Italia s/n, 11300 Montevideo, Uruguay.
³ Unidad de Medicina Intensiva, Hospital Maciel, Administración de los Servicios de Salud del Estado (ASSE), 25 de Mayo 174, 11000 Montevideo, Uruguay.
⁴ Cátedra de Medicina Intensiva, Hospital de Clínicas, Facultad de Medicina, Universidad de la República, Av Italia s/n, 11300 Montevideo, Uruguay.
⁵ Laboratorio de Citometria y Biologia Molecular, Departamento Básico de Medicina, Hospital de Clínicas, Facultad de Medicina, Universidad de la República, Av Italia s/n, 11300 Montevideo, Uruguay; Cátedra de Hematología, Hospital de Clínicas, Facultad de Medicina, Universidad de la República, Av Italia s/n, CP 11300 Montevideo, Uruguay. Electronic address: sgrille@hc.edu.uy.

PMID: 32623101
DOI: 10.1016/j.jneuroim.2020.577310

Abstract

To evaluate cerebrospinal fluid (CSF) and peripheral blood (PB) Treg, TH17 cells, TH1, TH2 and related cytokines in the acute phase of aSAH we assessed TH17, TH1, TH2, T regulatory cells and neutrophils in 39 aneurysmal subarachnoid hemorrhage (aSAH) patients and 56 controls. PB TH17 cells and TH17/Treg ratio were higher in CSF and PB of aSAH patients. Serum and CSF IL-17A levels were increased in aSAH. Serum IL-17A levels were associated with vasospasm and ICU mortality. Study results support the role of TH17/IL17 axis in aSAH pathogenesis, turning it into a potential clinical biomarker and a novel target for research.

Keywords: Aneurysmal subarachnoid hemorrhage; Flow cytometry; IL-17A; Neuroinflammation; T regulatory cells; TH17 cells.