Biotinylated recombinant interleukin-2. A tool for research on the interleukin-2 receptor

J Immunol Methods. 1988 Oct 26;113(2):221-9. doi: 10.1016/0022-1759(88)90335-3.


Recombinant interleukin-2 was biotinylated using a N-hydroxyl-succinimidyl [3H]biotin ester. The biotinylated lymphokine retained full binding and growth-promoting activities when assayed on the interleukin-2-dependent murine cell line HT-2. In preliminary studies, biotinylated interleukin-2 was used in conjunction with immunogold staining to demonstrate cell surface interleukin-2 receptors using both light and electron microscopy techniques. In addition, with rabbit anti-biotin antibodies, biotin-interleukin-2 was able to precipitate the 55 kDa IL-2 receptor from murine HT-2 cells. Thus, biotin-interleukin-2 represents a useful, non-radioactive tool for studying the structure and function of the interleukin-2 receptor.

MeSH terms

  • Animals
  • Biotin*
  • Cell Line
  • Electrophoresis, Polyacrylamide Gel
  • Immunohistochemistry* / methods
  • Interleukin-2* / metabolism
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / metabolism
  • Mice
  • Precipitin Tests / methods
  • Receptors, Interleukin-2 / analysis
  • Receptors, Interleukin-2 / isolation & purification*
  • Recombinant Proteins* / metabolism
  • Sodium Dodecyl Sulfate
  • T-Lymphocytes, Cytotoxic / analysis
  • T-Lymphocytes, Cytotoxic / ultrastructure


  • Interleukin-2
  • Membrane Proteins
  • Receptors, Interleukin-2
  • Recombinant Proteins
  • Sodium Dodecyl Sulfate
  • Biotin