Multiple biological activities are expressed by a mouse interleukin 6 cDNA clone isolated from bone marrow stromal cells

Proc Natl Acad Sci U S A. 1988 Oct;85(19):7099-103. doi: 10.1073/pnas.85.19.7099.


Interleukin 6 (IL-6) refers to the gene product that was characterized initially as beta 2 interferon/26-kDa protein produced by human fibroblasts and later was found to be identical to B-cell stimulatory factor 2, hybridoma/plasmacytoma growth factor, and probably hepatocyte-stimulating factor. Using the human IL-6 cDNA as a probe, we have isolated functional cDNA clones from mouse bone marrow stromal cell cDNA libraries. Sequence analysis of the mouse cDNA insert revealed significant homology between the human and mouse IL-6 cDNA clones both at the level of nucleotide (65%) and deduced amino acid (41%) sequences. The NH2-terminal sequence of the deduced protein is identical to a partial NH2-terminal sequence determined previously for a hybridoma/plasmacytoma growth factor and a plasmacytoma growth factor isolated from mouse T cells and macrophages, respectively. The mRNA for mouse IL-6 is expressed in IL-1-treated stromal cells and in activated T-cell and macrophage cell lines. Supernatants from COS-7 monkey cells transfected with the cDNA clone have plasmacytoma growth factor, hepatocyte-stimulating factor, and colony-stimulating factor activities, as well as the ability to support the growth of a factor-dependent myeloid cell line, thus revealing an additional biological activity for IL-6.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Bone Marrow / analysis*
  • Cell Line
  • Cloning, Molecular*
  • DNA / analysis*
  • Interleukin-6
  • Interleukins / genetics*
  • Mice
  • Molecular Sequence Data
  • RNA, Messenger


  • Interleukin-6
  • Interleukins
  • RNA, Messenger
  • DNA

Associated data

  • GENBANK/J03783