TLR13, TLR22, TRAF6, and TAK1 in the soiny mullet (Liza haematocheila): Molecular characterization and expression profiling analysis

Dev Comp Immunol. 2020 Nov:112:103774. doi: 10.1016/j.dci.2020.103774. Epub 2020 Jul 4.

Abstract

Toll-like receptors (TLRs) and their associated signaling pathways play pivotal roles in the immune response to invading pathogens. Here, TLR13, TLR22, tumor necrosis factor receptor-associated factor 6 (TRAF6), and transforming growth factor-β-activated kinase1 (TAK1) were characterized in the soiny mullet (Liza haematocheila), representative mugilid species that is widely cultured in Asia. The four mullet genes, which shared characteristic features with their counterparts in other teleosts, were ubiquitously expressed in all of the examined tissues, albeit with different expression patterns. Following Streptococcus dysgalactiae infection, the four genes were upregulated to different degrees in various mullet tissues. These results indicated that the four genes were involved in the mullet immune response to bacterial infection. To the best of our knowledge, this is the first characterization of these four genes in mullet. Our results provide a basis for future studies of TLR signaling pathways in mullet, as well as for similar studies in other mugilids.

Keywords: Bacterial infection; Expression; Liza haematocheila; TAK1; TRAF6; Toll-like receptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Asia
  • Cloning, Molecular
  • Fish Proteins / genetics*
  • Fish Proteins / metabolism
  • Fishes
  • Gene Expression Profiling
  • Immunity, Innate
  • MAP Kinase Kinase Kinase 7
  • MAP Kinase Kinase Kinases / genetics*
  • MAP Kinase Kinase Kinases / metabolism
  • Signal Transduction
  • Smegmamorpha / genetics*
  • Smegmamorpha / immunology
  • Streptococcal Infections / immunology*
  • Streptococcus / physiology*
  • TNF Receptor-Associated Factor 6 / genetics*
  • TNF Receptor-Associated Factor 6 / metabolism
  • Toll-Like Receptors / genetics*
  • Toll-Like Receptors / metabolism
  • Up-Regulation

Substances

  • Fish Proteins
  • MAP Kinase Kinase Kinases
  • TNF Receptor-Associated Factor 6
  • Toll-Like Receptors
  • MAP Kinase Kinase Kinase 7

Supplementary concepts

  • Streptococcus dysgalactiae