GC/MS analysis of Juniperus procera extract and its activity with silver nanoparticles against Aspergillus flavus growth and aflatoxins production

Biotechnol Rep (Amst). 2020 Jun 25;27:e00496. doi: 10.1016/j.btre.2020.e00496. eCollection 2020 Sep.

Abstract

From ancient to currently, it has been hard to prevent the exposure to mycotoxigenic fungi, due to these fungi occurs naturally in the environment. This paper reports the antifungal activities of the Juniperus procera stem extract with silver nanoparticles (AgNPs) against Aspergillus flavus growth and aflatoxins production. Numerous constituents of J. procera extract were detected by GC/MS analysis. Methanolic extract at 30, 60 and 90 mg/mL inhibited the growth of A. flavus, where the inhibition reached to 50.86, 51.60 and 52.58 %. respectively while weak inhibition was observed using the aqueous extract. Growth of A. flavus was reduced using AgNPs, the highest inhibition 39.31 % was recorded at 100 ppm AgNPs. Synergistic activity was observed by applying 50 ppm of AgNPs with aqueous and methanolic extracts of J. procera . A reduction in aflatoxin B2 and G2 synthesis was observed using different concentrations of methanolic stems extract of J. procera particularly with AgNPs.

Keywords: Aflatoxins; Juniperus procera; Mycotoxigenic fungi; Silver nanoparticles.