Discovery and modification of cytochrome P450 for plant natural products biosynthesis

Xiaonan Liu; Xiaoxi Zhu; Hui Wang; Tian Liu; Jian Cheng; Huifeng Jiang

doi:10.1016/j.synbio.2020.06.008

Discovery and modification of cytochrome P450 for plant natural products biosynthesis

Synth Syst Biotechnol. 2020 Jul 1;5(3):187-199. doi: 10.1016/j.synbio.2020.06.008. eCollection 2020 Sep.

Authors

Xiaonan Liu^{1

2}, Xiaoxi Zhu^{1

2}, Hui Wang³, Tian Liu^{4

1}, Jian Cheng¹, Huifeng Jiang¹

Affiliations

¹ Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
² University of Chinese Academy of Sciences, Beijing, 100049, China.
³ College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, China.
⁴ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.

Abstract

Cytochrome P450s are widespread in nature and play key roles in the diversification and functional modification of plant natural products. Over the last few years, there has been remarkable progress in plant P450s identification with the rapid development of sequencing technology, "omics" analysis and synthetic biology. However, challenges still persist in respect of crystal structure, heterologous expression and enzyme engineering. Here, we reviewed several research hotspots of P450 enzymes involved in the biosynthesis of plant natural products, including P450 databases, gene mining, heterologous expression and protein engineering.

Keywords: P450 enzymes; Plant natural products; Synthetic biology.