Investigating the transmission profiles of 808 nm laser through different regions of the rat's head

Lasers Med Sci. 2021 Jun;36(4):803-810. doi: 10.1007/s10103-020-03098-9. Epub 2020 Jul 8.


Studying light penetration in biological tissues became a very important concern in various medical applications. It is an essential factor required to resolve the optical dose in many diagnostic and therapeutic procedures. The absorption and scattering properties of the inspected tissue control how deep the light will travel inside the tissue. However, these optical properties are highly dependent on the wavelength of the light source. In this work, the light transmission through different regions of the rat's head was investigated and the minimum laser power required to reach different parts of the head is also determined using 808-nm semiconductor laser diode. The power variation in different regions of the head is estimated using Monte Carlo simulation. Absorption and scattering coefficients of the head layers were calculated using integrating sphere measurements and Kubelka-Munk model. The absorption coefficient of the skin was 0.19 ± 0.071 mm-1, 0.024 ± 0.11 mm-1 for skull, and 0.35 ± 0.13 mm-1 for the brain, while the scattering coefficients were 7.35 ± 1.09, 2.71 ± 0.37, and 13.04 ± 0.36 mm-1 for skin, skull, and brain, respectively. The obtained results provide a relationship between laser incident power and the depth in the rat's head showing a higher optical transmission at the frontal part of the head than the middle or back regions due to the variations in the skull thickness. Therefore, the study revealed that the transmitted power of 808 nm laser at different incident locations on the head is nonlinear and variable due to different skull's thickness.

Keywords: Optical transmission; Rat’s head; Tissue scattering; Transcranial laser irradiation.

MeSH terms

  • Animals
  • Brain / radiation effects
  • Computer Simulation
  • Head / radiation effects*
  • Lasers, Semiconductor*
  • Monte Carlo Method*
  • Optical Phenomena*
  • Rats
  • Scattering, Radiation
  • Skull / radiation effects