Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties

G Amicosante; A Oratore; N Franceschini; M Maccarrone; R Strom; M Galleni; J M Frère

doi:10.1042/bj2540885

Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties

Biochem J. 1988 Sep 15;254(3):885-90. doi: 10.1042/bj2540885.

Authors

G Amicosante¹, A Oratore, N Franceschini, M Maccarrone, R Strom, M Galleni, J M Frère

Affiliation

¹ Università degli Studi dell'Aquila, Dipartimento di Scienze e Tecnologie Biomediche e di Biometria, Italy.

Abstract

Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat: values similar to those observed with the best penicillin substrates. Cloxacillin, methicillin and imipenem were hydrolysed very slowly. Hydrolysis of azthreonam could not be detected.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids / analysis
Cephalosporins / metabolism
Chromatography, Affinity
Chromosomes, Bacterial / enzymology
Citrobacter / enzymology*
Electrophoresis, Polyacrylamide Gel
Isoelectric Point
Isoenzymes / isolation & purification*
Kinetics
Molecular Weight
Penicillins / metabolism
Substrate Specificity
beta-Lactamases / isolation & purification*

Substances

Amino Acids
Cephalosporins
Isoenzymes
Penicillins
beta-Lactamases