Amino acid sequence and domain structure of entactin. Homology with epidermal growth factor precursor and low density lipoprotein receptor

J Cell Biol. 1988 Dec;107(6 Pt 2):2749-56. doi: 10.1083/jcb.107.6.2749.

Abstract

Entactin (nidogen), a 150-kD sulfated glycoprotein, is a major component of basement membranes and forms a highly stable noncovalent complex with laminin. The complete amino acid sequence of mouse entactin has been derived from sequencing of cDNA clones. The 5.9-kb cDNA contains a 3,735-bp open reading frame followed by a 3'-untranslated region of 2.2 kb. The open reading frame encodes a 1,245-residue polypeptide with an unglycosylated Mr of 136,500, a 28-residue signal peptide, two Asn-linked glycosylation sites, and two potential Ca2+-binding sites. Analysis of the deduced amino acid sequence predicts that the molecule consists of two globular domains of 70 and 36 kD separated by a cysteine-rich domain of 28 kD. The COOH-terminal globular domain shows homology to the EGF precursor and the low density lipoprotein receptor. Entactin contains six EGF-type cysteine-rich repeat units and one copy of a cysteine-repeat motif found in thyroglobulin. The Arg-Gly-Asp cell recognition sequence is present in one of the EGF-type repeats, and a synthetic peptide from the putative cell-binding site of entactin was found to promote the attachment of mouse mammary tumor cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Basement Membrane / analysis*
  • Cell Adhesion
  • Cell Line
  • Cloning, Molecular
  • DNA / genetics
  • Epidermal Growth Factor / genetics*
  • Glycoproteins / analysis
  • Glycoproteins / genetics*
  • Membrane Glycoproteins*
  • Mice
  • Molecular Sequence Data
  • Protein Precursors / genetics*
  • Receptors, LDL / genetics*
  • Sequence Homology, Nucleic Acid

Substances

  • Glycoproteins
  • Membrane Glycoproteins
  • Protein Precursors
  • Receptors, LDL
  • epidermal growth factor precursor
  • nidogen
  • Epidermal Growth Factor
  • DNA

Associated data

  • GENBANK/X14194