Effects of Percutaneous Collagen Induction Therapy Associated with Hyaluronic Acid on Inflammatory Response, Oxidative Stress, and Collagen Production

Inflammation. 2020 Dec;43(6):2232-2244. doi: 10.1007/s10753-020-01291-0.


Percutaneous collagen induction (PCI) is an alternative treatment for skin dysfunctions, it aims to stimulate collagen production by encouraging normal wound healing that occurs after any trauma by inducing microlesions; also it may be potentiated with the association with drugs such as hyaluronic acid (HA). Our objective was to evaluate the effects of PCI associated with hyaluronic acid (0.9%) on inflammatory process, oxidative stress, and collagen production in rat epidermis. For the study, 36 adult Wistar rats were randomly divided into 6 groups (n = 6): Control; PCI 0.5; PCI 1.0; HA; PCI 0.5 + HA; and PCI 1.0 + HA. The animals were anesthetized, trichotomized, and the application of therapies was performed once; After 7 days, the animals were euthanized for removal of the skin region. Levels of pro-inflammatory (IL1, IL6, TNFα), anti-inflammatory (IL4 and IL10) cytokines and growth factors (FGF, TGFβ) were evaluated, besides oxidative stress parameters and histological analysis. In combination groups, there is a decrease in TNFα compared with the control and PCI groups in contrast to a significant increase in anti-inflammatory cytokines and growth factors. Oxidant and oxidative damage levels showed a significant decrease in PCI + HA groups in relation to PCI groups while antioxidant defense increased in PCI + HA groups compared with the control group. The number of fibroblasts was increased in the PCI 1.0 group in relation to the control, HA, and PCI 0.5. The number of blood vessels and collagen area was increased in groups PCI and PCI + HA compared with the HA group. We conclude that the combination of PCI with HA is able to accelerate the acute inflammatory process, reducing its deleterious effects and anticipating the chronic response, contributing to tissue repair.

Keywords: collagen; cytokines; hyaluronic acid; inflammation; oxidative stress; percutaneous collagen induction.

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Collagen / metabolism*
  • Cytokines / metabolism
  • Fibroblasts / metabolism
  • Hyaluronic Acid / metabolism*
  • Induction Chemotherapy
  • Inflammation*
  • Male
  • Oxidative Stress*
  • Percutaneous Coronary Intervention
  • Rats
  • Rats, Wistar
  • Reactive Oxygen Species
  • Wound Healing / drug effects


  • Antioxidants
  • Cytokines
  • Reactive Oxygen Species
  • Hyaluronic Acid
  • Collagen