Development of a monoclonal antibody-based antigen capture enzyme-linked immunosorbent assay for detection of H7N9 subtype avian influenza virus

J Med Virol. 2021 Jun;93(6):3939-3943. doi: 10.1002/jmv.26292. Epub 2020 Jul 27.

Abstract

To establish a rapid detection method for H7N9 avian influenza virus (AIV), monoclonal antibodies (mAbs) against hemagglutinin (HA) of H7N9 were developed to establish an antigen-capture enzyme-linked immunosorbent assay (AC-ELISA). AC-ELISA achieved high specificity and sensitivity, with a detection limit of 3.9 ng/mL for H7N9 HA protein (A/Zhejiang/DTID-ZJU01/2013), and 2-2 HA unit/100 μL for live H7N9 AIV. The inter- and intra-assay coefficient of variation was less than 10%. Compared with conventional virus isolation detection, the sensitivity and specificity were 94.96% and 88.24%, respectively. AC-ELISA proved to be a rapid and practical technique for the detection of H7N9 AIV.

Keywords: H7N9; antigen-capture enzyme-linked immunosorbent assay; assay sensitivity; avian influenza virus; monoclonal antibody; virus detection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Viral / immunology
  • Birds / virology
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Enzyme-Linked Immunosorbent Assay / standards
  • Humans
  • Influenza A Virus, H7N9 Subtype / immunology*
  • Influenza, Human / diagnosis*
  • Influenza, Human / virology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Orthomyxoviridae Infections / diagnosis
  • Orthomyxoviridae Infections / immunology*
  • Orthomyxoviridae Infections / virology
  • Sensitivity and Specificity

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral