Achieving Nonenzymatic Blood Glucose Sensing by Uprooting Saturation

Anal Chem. 2020 Aug 4;92(15):10777-10782. doi: 10.1021/acs.analchem.0c02218. Epub 2020 Jul 22.


The saturation of nonenzymatic blood glucose sensors at lower than normal blood glucose levels has blocked their practical applications. The mechanistic understanding of the saturation, however, has long been under debate. Employing cyclic voltammetry, amperometry, and FTIR with various electrolytes of varying concentrations, we were able to uproot the saturation cause. It was found to be related to the hydroxide ion concentration, which must be 11 times greater than that of the glucose concentration, contrary to the prior understanding. Together with the satisfactory sensitivity at high pH, nonenzymatic blood glucose sensing has finally been achieved, eliminating the usual problem of electrochemical current saturation as well as the need for enzyme found in the present technology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Chemical Analysis / instrumentation
  • Blood Chemical Analysis / methods*
  • Blood Glucose / analysis*
  • Electrochemistry
  • Electrodes
  • Hydrogen-Ion Concentration
  • Limit of Detection
  • Spectroscopy, Fourier Transform Infrared


  • Blood Glucose