Genome sequencing and transcriptome analysis of Geotrichum citri-aurantii on citrus reveal the potential pathogenic- and guazatine-resistance related genes

Genomics. 2020 Nov;112(6):4063-4071. doi: 10.1016/j.ygeno.2020.07.013. Epub 2020 Jul 7.

Abstract

Sour rot, caused by Geotrichum citri-aurantii, is a major postharvest disease of citrus，and it causes serious economic losses. In this study, a high-quality genome sequence of G. citri-aurantii was obtained by Single Molecule Real-Time Sequencing (SMRT). Approximately 5.43 Gb of clean data were obtained and a total of 27.94-Mb genomic sequence was mapped to 10 chromosome groups after high-through chromosome conformation capture (Hi-C) assembly. In addition, three polygalacturonase genes which were related to pathogenicity in G. citri-aurantii genome were discovered. And transcriptome data of guazatine-resistance had been analyzed, the results showed that the guazatine-resistance of G. citri-aurantii was related to two ATP-binding cassette (ABC) transporter family genes, six major facilitator superfamily (MFS) transporter family genes and two multidrug and toxic compound extrusion (MATE) transporter family genes. In summary, our research may provide novel insights into the effective control of this pathogen.

Keywords: Citrus; Gene; Genome; Geotrichum citri-aurantii; Guazatine; Polygalacturonase; Transcriptome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Citrus / microbiology*
  • Drug Resistance, Fungal / genetics
  • Fungicides, Industrial / pharmacology*
  • Gene Expression Profiling
  • Genes, Fungal
  • Genome, Fungal*
  • Genomics
  • Geotrichum / drug effects
  • Geotrichum / genetics*
  • Geotrichum / metabolism
  • Geotrichum / pathogenicity
  • Guanidines / pharmacology*
  • Membrane Transport Proteins / genetics
  • Polygalacturonase / genetics

Substances

  • Fungicides, Industrial
  • Guanidines
  • Membrane Transport Proteins
  • guazatine
  • Polygalacturonase

Supplementary concepts

  • Geotrichum citri-aurantii