A single nucleotide substitution in TaHKT1;5-D controls shoot Na+ accumulation in bread wheat

Plant Cell Environ. 2020 Sep;43(9):2158-2171. doi: 10.1111/pce.13841. Epub 2020 Jul 22.


Improving salinity tolerance in the most widely cultivated cereal, bread wheat (Triticum aestivum L.), is essential to increase grain yields on saline agricultural lands. A Portuguese landrace, Mocho de Espiga Branca accumulates up to sixfold greater leaf and sheath sodium (Na+ ) than two Australian cultivars, Gladius and Scout, under salt stress in hydroponics. Despite high leaf and sheath Na+ concentrations, Mocho de Espiga Branca maintained similar salinity tolerance compared to Gladius and Scout. A naturally occurring single nucleotide substitution was identified in the gene encoding a major Na+ transporter TaHKT1;5-D in Mocho de Espiga Branca, which resulted in a L190P amino acid residue variation. This variant prevents Mocho de Espiga Branca from retrieving Na+ from the root xylem leading to a high shoot Na+ concentration. The identification of the tissue-tolerant Mocho de Espiga Branca will accelerate the development of more elite salt-tolerant bread wheat cultivars.

Keywords: Na+ transport; plant growth; salt tolerance; sodium; xylem sap Na+.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Female
  • Gene Expression Regulation, Plant
  • Models, Molecular
  • Oocytes / metabolism
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plant Shoots / genetics
  • Plant Shoots / metabolism*
  • Polymorphism, Single Nucleotide
  • Potassium-Hydrogen Antiporters / chemistry
  • Potassium-Hydrogen Antiporters / genetics
  • Potassium-Hydrogen Antiporters / metabolism
  • Salt Tolerance / genetics
  • Sodium / metabolism*
  • Triticum / genetics*
  • Triticum / metabolism*
  • Xenopus laevis
  • Xylem / genetics
  • Xylem / metabolism


  • Plant Proteins
  • Potassium-Hydrogen Antiporters
  • Sodium