Evaluation of the new differential somatic cell count parameter as a rapid and inexpensive supplementary tool for udder health management through regular milk recording

Abstract

Mastitis, particularly in its subclinical form, is the costliest disease in milk production causing substantial financial losses to the dairy industry, impairing animal welfare, and one of the main reasons for treating dairy cows with antimicrobials. Somatic cell count (SCC) is broadly used as an indicator for mastitis or intramammary infection (IMI) and is the basis for udder health management programmes, e.g., through monthly dairy herd improvement (DHI) testing. While SCC shows the total number of cells in milk, the new Differential SCC (DSCC) shows also the combined proportion of polymorphonuclear neutrophils (PMN) and lymphocytes as a percentage of the total SCC. In this study, we investigated the test characteristics of DSCC as a new supplementary indicator for mastitis screening. We collaborated with 11 herds totalling 969 dairy cows and collected metered DHI samples once a month over four months. The IMI status was assessed through analysis of aseptic composite hand-stripped samples using culture and followed by species identification using MALDI-ToF. The pathogens detected were categorised as 'no', 'minor', 'major', or 'other' pathogens. The results of our study showed that the DSCC parameter was significantly associated with the IMI status and the cow's parity but not with days in milk or test-day milk weight. On the other hand, SCC was associated with all these four factors. DSCC counts were significantly higher in samples of cows with IMI caused by major pathogens as compared to cows with no IMI or IMI by minor or other pathogens. SCC alone, DSCC alone, and the combination of DSCC and SCC were further compared based on test characteristics using exemplary cut-offs. For example, working with a cut-off of 200,000 cells/ for SCC alone compared to working with the combination of DSCC of 65 % and/or 200,000 cells/mL to classify cows as infected by major pathogens, the sensitivity increased from 78 % to 92 % and the specificity decreased from 87 % to 66 %. With the combination, the positive predictive value changed from 52 % to 34 %, and the negative predictive value stayed at the same level (96 % vs 98 %). In summary, our study provides first insights on test characteristics of the DSCC parameter used in combination with the well-established SCC for monitoring udder health using DHI testing. This combination opens up the possibility to further improve udder health monitoring programmes (e.g., improved identification of IMI caused by major pathogens) but more work on the subject is needed.

Keywords: Dairy herd improvement; Differential somatic cell count; Intramammary infection; Mastitis; Test characteristics; Udder health.