IS Kpn40-Mediated Mobilization of the Colistin Resistance Gene mcr-3.11 in Escherichia coli

Yu-Zhang He; Teng-Fei Long; Cai-Ping Chen; Bing He; Xing-Ping Li; Jessica Schuyler; Liang Chen; Xiao-Ping Liao; Ya-Hong Liu; Jian Sun

doi:10.1128/AAC.00851-20

IS Kpn40-Mediated Mobilization of the Colistin Resistance Gene mcr-3.11 in Escherichia coli

Antimicrob Agents Chemother. 2020 Sep 21;64(10):e00851-20. doi: 10.1128/AAC.00851-20. Print 2020 Sep 21.

Authors

Yu-Zhang He^{1

2

3}, Teng-Fei Long^{1

2

3}, Cai-Ping Chen^{1

2

3}, Bing He^{1

2

3}, Xing-Ping Li^{1

2

3}, Jessica Schuyler^{4

5}, Liang Chen^{4

6}, Xiao-Ping Liao^{1

2

3}, Ya-Hong Liu^{1

2

3

5}, Jian Sun^{7

2

3}

Affiliations

¹ National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, People's Republic of China.
² Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, Guangdong, People's Republic of China.
³ Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, People's Republic of China.
⁴ Hackensack-Meridian Health Center for Discovery and Innovation, Nutley, New Jersey, USA.
⁵ Jiangsu Co-Innovation Centre for Prevention and Control of Important Animal Infectious, Diseases and Zoonoses, Yangzhou, Jiangsu, People's Republic of China.
⁶ Hackensack Meridian School of Medicine, Seton Hall University, Nutley, New Jersey, USA.
⁷ National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, People's Republic of China jiansun@scau.edu.cn.

Abstract

The mobile colistin resistance gene mcr-3 has globally disseminated since it was first reported in 2017 in Escherichia coliIn vitro mobilization assays in this study demonstrate the functionality of the composite transposon structure ISKpn40-mcr-3.11-dgkA-ISKpn40 in wild-type and recA^-E. coli strains. These transpositions generated 4-bp duplications at the target sites. This is the first report demonstrating the mobility of the mcr-3.11 gene by transposition.

Keywords: ISKpn40; colistin resistance; mcr-3.11; transposition; transposition mechanism.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Colistin* / pharmacology
Drug Resistance, Bacterial* / genetics
Escherichia coli / genetics
Escherichia coli Proteins* / genetics
Microbial Sensitivity Tests
Plasmids / genetics
Transferases (Other Substituted Phosphate Groups)

Substances

Anti-Bacterial Agents
Escherichia coli Proteins
MCR-3 protein, E coli
Transferases (Other Substituted Phosphate Groups)
Colistin