Abstract
We report a novel approach for magneto-controlled activation of an artificial electro-enzymatic cascade. The input signal triggers release of a caged ligand peptide, its proteolytic processing and activation of an artificial allosteric enzyme based on PQQ-dependent glucose dehydrogenase. The developed cascade was used to assemble a magneto-controlled biofuel cell.
MeSH terms
-
Alanine Transaminase / chemistry
-
Allosteric Regulation
-
Amino Acid Oxidoreductases / chemistry
-
Bioelectric Energy Sources*
-
Calmodulin / chemistry*
-
Calmodulin-Binding Proteins / chemistry*
-
Electrochemical Techniques / instrumentation
-
Electrochemical Techniques / methods
-
Electrodes
-
Enzymes, Immobilized / chemistry
-
Glucose / chemistry
-
Glucose Dehydrogenases / chemistry*
-
Magnetic Fields
-
Magnetite Nanoparticles / chemistry*
-
Nanotubes, Carbon / chemistry
-
Oxidoreductases Acting on CH-CH Group Donors / chemistry
-
Recombinant Fusion Proteins / chemistry
Substances
-
Calmodulin
-
Calmodulin-Binding Proteins
-
Enzymes, Immobilized
-
Magnetite Nanoparticles
-
Nanotubes, Carbon
-
Recombinant Fusion Proteins
-
Glucose Dehydrogenases
-
glucose dehydrogenase (pyrroloquinoline-quinone)
-
Oxidoreductases Acting on CH-CH Group Donors
-
bilirubin oxidase
-
Amino Acid Oxidoreductases
-
L-glutamate oxidase
-
Alanine Transaminase
-
Glucose