Local administration of cells containing an inserted IL-2 gene and producing IL-2 inhibits growth of human tumours in nu/nu mice

Immunol Lett. 1988 Dec;19(4):279-82. doi: 10.1016/0165-2478(88)90155-1.


We have prepared a retroviral expression construct, pPS-IL-2, in which human IL-2 cDNA has been inserted into the polylinker region, and have used the retroviral vector to introduce the functional IL-2 gene into a fibroblast cell line, RAT-1. Peritumoral administration of IL-2-producing RAT-1 cells into congenitally athymic (nu/nu) mice carrying subcutaneous transplants of human carcinoma cells inhibited the growth of the human tumour xenografts.

MeSH terms

  • Animals
  • Fibroblasts / immunology
  • Fibroblasts / transplantation
  • Genetic Engineering*
  • Genetic Vectors
  • HeLa Cells / immunology
  • HeLa Cells / pathology
  • Humans
  • Interleukin-2 / biosynthesis
  • Interleukin-2 / genetics*
  • Killer Cells, Natural / immunology
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Neoplasms, Experimental / genetics
  • Neoplasms, Experimental / immunology
  • Neoplasms, Experimental / therapy*
  • Retroviridae / genetics
  • Transformation, Genetic


  • Interleukin-2