Molecular investigation of allelic variants of EqCXCL16 gene in equine arteritis virus infected stallions of selected horse breeds in Poland

Infect Genet Evol. 2020 Nov:85:104455. doi: 10.1016/j.meegid.2020.104455. Epub 2020 Jul 12.


Susceptibility to long-term persistent infection with Equine Arteritis Virus (EAV) in stallions is related with EqCXCL16 gene alleles of the host. In our study EqCXCL16 gene alleles were determined for 63 EAV shedders and 126 non-shedders of various horse breeds. In total, 60 (31.7%) out of 189 tested stallions were identified as carriers of susceptible variants of EqCXCL16 by real time PCR and Sanger sequencing. The presence of susceptible genotype was related to horse breed with the highest percentage in Wielkopolska breed, Polish coldblood and Silesian breed horses. Strong correlation between EqCXCL16 susceptible genotypes and EAV shedding in semen (p < .0001) was observed.

Keywords: EqCXCL16; Equine arteritis virus; Persistent infection; Stallions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Arterivirus Infections / veterinary*
  • Arterivirus Infections / virology*
  • Chemokine CXCL16 / genetics*
  • Equartevirus / genetics*
  • Genotype
  • Horse Diseases / virology*
  • Horses / virology*
  • Phylogeny
  • Poland / epidemiology
  • RNA, Viral
  • Semen / virology
  • Sequence Analysis


  • Chemokine CXCL16
  • RNA, Viral