Thioglycoligation of aromatic thiols using a natural glucuronide donor

Org Biomol Chem. 2020 Aug 7;18(29):5582-5585. doi: 10.1039/d0ob00226g. Epub 2020 Jul 16.


The β-d-glucuronidase DtGlcA from Dictyoglomus thermophilum was engineered to generate an active thioglycoligase that is able to catalyse the formation of numerous S-glucuronides. Its X-ray structure analysis indicated the ability of the biocatalyst to bind aromatic thiol acceptors for S-glycosylation. Noteworthily, the DtGlcA mutant was found to be the first thioligase that is able to use a natural sugar donor different from the widely used synthetic para-nitrophenyl glycosides.

Publication types

  • Research Support, Non-U.S. Gov't