Cooling assisted headspace microextraction by packed sorbent coupled to HPLC for the determination of volatile polycyclic aromatic hydrocarbons in soil

Fariba Nazari Serenjeh; Payman Hashemi; Ali Reza Ghiasvand; Fahimeh Rasolzadeh; Nahid Heydari; Alireza Badiei

doi:10.1016/j.aca.2020.05.067

Cooling assisted headspace microextraction by packed sorbent coupled to HPLC for the determination of volatile polycyclic aromatic hydrocarbons in soil

Anal Chim Acta. 2020 Aug 15:1125:128-134. doi: 10.1016/j.aca.2020.05.067. Epub 2020 May 29.

Authors

Fariba Nazari Serenjeh¹, Payman Hashemi², Ali Reza Ghiasvand², Fahimeh Rasolzadeh², Nahid Heydari², Alireza Badiei³

Affiliations

¹ Department of Chemistry, Faculty of Science, Lorestan University, Khoramabad, Iran. Electronic address: nazari.fa@fs.lu.ac.ir.
² Department of Chemistry, Faculty of Science, Lorestan University, Khoramabad, Iran.
³ School of Chemistry, College of Science, University of Tehran, Tehran, Iran.

PMID: 32674758
DOI: 10.1016/j.aca.2020.05.067

Abstract

A headspace microextraction by packed sorbent technique (HS-MEPS) was developed that is assisted by cooling the extraction phase and, in the same time, heating the sample matrix. The innovated cooling assisted HS-MEPS system was optimized for the extraction and HPLC determination of volatile polycyclic aromatic hydrocarbons (PAHs) in solid samples. Amino ethyl-functionalized SBA-15 was used as a nanoporous sorbent for packing the conical hub of the MEPS syringe. For efficient extraction of PAHs, several parameters such as the nature and mass of sorbent, eluent type, elution volume, extraction temperature and number of draw-eject cycles of the syringe were studied and optimized. Under the optimal experimental conditions, only 2 mg of the sorbent was sufficient by applying a temperature of 0 °C to the sorbent while the sample was heated up to 150 °C. Linear calibration curves (R² > 0.95) were obtained for all the studied PAHs and their quantitation limits were in the range of 0.042-0.25 ng g^-1. The method was successfully applied to the extraction and HPLC analysis of PAHs in some polluted soil samples.

Keywords: Amino ethyl-functionalized SBA-15; Cooling assisted headspace microextraction by packed sorbent; High performance liquid chromatography; Polycyclic aromatic hydrocarbons.