Controllable release of dopamine from simulated enzyme-containing biomembrane by biased potential

Abstract

Controllable release of dopamine (DA) is worth studying for its significant in physiological metabolic process. β-cyclodextrin/11-mercaptoundecanoic acid self-assembled monolayer (β-CD/MUA SAM) based on hydrogen bonds network was constructed as simulated enzyme-containing biomembrane. DA interacted with β-CD based on intermolecular hydrogen bond and formed inclusion complexes in SAM, namely DA@β-CD/MUA SAM. The desorption process of DA from DA@β-CD/MUA SAM revealed the release behavior of DA from enzyme-containing biomembrane at molecular level. Positive biased potential as external stimulus was applied at DA@β-CD/MUA SAM, reducing the negative charge density of SAM. The decrease of negative charge density of SAM resulted in the weakening of hydrogen bond between DA and β-CD, which in turn caused DA to be released. Using scanning electrochemical microscopy, positive biased potential not only had specificity induction for the release of DA, but also was able to monitor the release quantitatively in real time. These results showed that positive biased potential as external stimulus was favorable for the controllable release of DA, suggesting the possible application of biased potential in controllable regulation field.

Keywords: Biased potential; Controllable release; Dopamine; Hydrogen bond; Quantitatively.