DETECTION OF MYCOBACTERIUM TUBERCULOSIS DNA IN BUCCAL SWAB SAMPLES FROM CHILDREN IN LIMA, PERU

Pediatr Infect Dis J. 2020 Jul 14. doi: 10.1097/INF.0000000000002828. Online ahead of print.

Abstract

We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%-42%) in 24 culture-confirmed tuberculosis cases and 4.6% (95% CI: 1%-13%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99% in the 199 controls (95% CI: 96%-100%).