Advances in methods for atomic resolution macromolecular structure determination

F1000Res. 2020 Jul 2:9:F1000 Faculty Rev-667. doi: 10.12688/f1000research.25097.1. eCollection 2020.


Recent technical advances have dramatically increased the power and scope of structural biology. New developments in high-resolution cryo-electron microscopy, serial X-ray crystallography, and electron diffraction have been especially transformative. Here we highlight some of the latest advances and current challenges at the frontiers of atomic resolution methods for elucidating the structures and dynamical properties of macromolecules and their complexes.

Keywords: Structural biology; computational methods; electron diffraction; electron microscopy; x-ray crystallography.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Cryoelectron Microscopy
  • Crystallography, X-Ray
  • Macromolecular Substances / chemistry*
  • Molecular Structure*


  • Macromolecular Substances