Saccharomyces cerevisiae and Lactobacillus rhamnosus cell walls immobilized on nano-silica entrapped in alginate as aflatoxin M1 binders

Abstract

Aflatoxins are common fungal toxins in foods that cause health problems for humans. The aim of this study was to use Saccharomyces cerevisiae and Lactobacillus rhamnosus cell walls immobilized on nano-silica entrapped in alginate as aflatoxin M₁ (AFM₁) binders. In this study, microbial walls were disrupted using a three-step mechanical technique including autoclave, thermal shock, and ultrasound. Dynamic light scattering (DLS) results proved size reduction in microbial walls ranging 75.8-91.4 nm. Disrupted walls were immobilized on nano-silica to enhance the efficiency of AFM₁ adsorption. Then, to prevent the release of the nano-silica or cell walls into the reaction medium, they were entrapped into alginate gel beads. Fourier transform infrared spectrometer (FT-IR) and scanning electron microscopy (SEM) micrographs confirmed the immobilization and entrapment process. Individual and mixtures of free cell walls, immobilized-entrapped walls, alginate bead and nano-silica were contacted with AFM₁ for 15 min and 24 h. AFM₁ reduction ability was evaluated using high performance liquid chromatography (HPLC). The results showed an AFM₁ reduction ranging 53-87% for free cell walls mixture at 15 min and alginate bead respectively. Also, it was possible to reuse immobilized-entrapped walls as binders with an efficiency of about 85%.

Keywords: Aflatoxin M(1); Alginate; Cell wall; Lactobacillus rhamnosus; Nano-silica; Saccharomyces cerevisiae.