Roles of hsa-miR-12462 and SLC9A1 in acute myeloid leukemia

J Hematol Oncol. 2020 Jul 23;13(1):101. doi: 10.1186/s13045-020-00935-w.


MicroRNAs (miRNAs) play important roles in cell proliferation, differentiation, and survival and may be useful for acute myeloid leukemia (AML) diagnosis and prognosis. In this study, we defined a novel miRNA, hsa-miR-12462, through small RNA sequencing of the bone marrow (BM) cells from 128 AML patients. Overexpression of hsa-miR-12462 in AML cells (U937 and HL-60) significantly decreased their growth rate when compared with those of the wild-type and MOCK controls. In a xenograft mouse model, tumor weight and size in the mice bearing the U937 cells with hsa-miR-12462 overexpression were significantly reduced when compared with those bearing the mock cells. The AML cells overexpressing hsa-miR-12462 had increased sensitivity to cytarabine chemotherapy. Combining the data from the MiRDB, an online microRNA database ( ), with the RNA-sequencing results, SLC9A1 was predicted to be one of the targets of hsa-miR-12462. hsa-miR-12462 was further confirmed to bind exclusively to the 3'UTR of SLC9A1 in U937 cells, leading to downregulation of SLC9A1. In summary, a higher level of hsa-miR-12462 in AML cells is associated with increased sensitivity to cytarabine chemotherapy via downregulation of SLC9A1.

Keywords: Acute myeloid leukemia; RNA sequencing; SLC9A1; miRNA.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Cytarabine / pharmacology
  • Drug Resistance, Neoplasm
  • Gene Expression Regulation / drug effects
  • HL-60 Cells
  • Humans
  • Leukemia, Myeloid, Acute / genetics*
  • Mice
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / physiology*
  • RNA, Neoplasm / genetics*
  • Sodium-Hydrogen Exchanger 1 / genetics
  • Sodium-Hydrogen Exchanger 1 / physiology*
  • Tumor Burden
  • Tumor Cells, Cultured
  • U937 Cells
  • Xenograft Model Antitumor Assays


  • 3' Untranslated Regions
  • Neoplasm Proteins
  • RNA, Neoplasm
  • SLC9A1 protein, human
  • Sodium-Hydrogen Exchanger 1
  • Cytarabine